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Dendritic polymers can be loaded with DNA, peptides, or proteins into cells by endocytosis, in which DNA is released into the nucleus for gene integration. Alfa Cytology provides high-quality dendrimer delivery system development services to safeguard our clients' vaccine projects.
Dendrimers are hyperbranched polymers that can load or conjugate peptide antigens and immune-stimulating molecules. For example, polyamide-amine (PAMAM) and polyethyleneimine (PEI) have highly branched structures, positive charge, and abundant peripheral functional groups, which show natural competitiveness in antigen delivery and vaccine construction. PAMAM dendrimers conjugated with MHC Class II peptides can target APCs and are used to deliver nucleic acids. Mannose-modified sugar dendrimer can improve the cellular uptake of APC.
The modified dendrimer can be used as an effective protein carrier to enable protein antigen delivery, resulting in effective antigen cross-presentation by the dendritic cell (DC). Targeting different customer needs, Alfa Cytology provides diversified dendrimer delivery systems development services, including but not limited to the following.
Functionalized Dendrimers Delivery Systems Development Service
Polyethylene glycol (PEG) can extend the blood circulation time of nanomaterials. Micelles constructed from PEG and cation dendrimer are ideal carriers for TAAs to improve their delivery in vivo. Dendritic molecules can also be used in combination with inorganic or polymer nanoparticles as functionalized molecules, which can improve the dispersion of nanoparticles and also promote cell uptake.
Peptide Dendrimer Delivery Systems Development Service
The use of dendritic cationic hydrophilic antimicrobial peptide as a delivery vector can enhance the antitumor effect of antigen-loaded DC vaccine. In addition, it also has multiple functions as an immune adjuvant and dendritic cell migration promoter, which can effectively improve the efficiency of antigen-presenting cells (APC) uptake and cross-presentation of antigens, and deliver antigens to lymph nodes through APC.
Dendrimer-Based Carriers for Protein/Peptide Cancer Vaccines
Since guanidine ligands readily bind proteins via strong hydrogen bonds and salt Bridges, modification of guanidine benzoic acid to dendritic polymers can enhance protein or peptide loading and delivery, further promoting a stronger DC maturation effect. Chemical modifications to form protein surfaces can also resist extracellular antigen release and trigger antigen delivery.
Dendrimer-Based Carriers for Nucleic Acid Cancer Vaccines
Nucleic acid-based vaccines load several different plasmids (pDNA) and targeted peptides onto dendrimers. It can selectively target APCs and efficiently deliver pDNA-encoding tumor antigens into APCs and induce high affinity of cytotoxic T lymphocytes (CTL) to tumor cells and anti-tumor humoral response. The dendrimer is conjugated with a lipid tail to form a cationic lipid, which is mixed with mRNA to prepare a vaccine by self-assembly. DCs can successfully take up mRNA with high transfection efficiency. Not only can it be used to deliver mRNA, but it can also be used to stimulate the Toll-like receptor 4 (TLR 4) signaling pathway in DC, thereby promoting the expression of multiple inflammatory cytokines, thereby inhibiting the occurrence of tumors.
We have extensive experience in the field of cancer vaccine development.
We have advanced laboratory facilities and technology platforms.
We can provide customized solutions based on specific customer needs and project objectives.
We are committed to providing high-quality research results and reliable data support.
Dendrimer delivery systems can be used for peptide vaccines, nucleic acid vaccines, whole-cell vaccines, etc. Alfa Cytology is committed to solving various problems in the field of cancer vaccines and has in-depth and extensive preclinical experience. If you are interested in our service, please feel free to contact us for more details.